Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element.
نویسندگان
چکیده
منابع مشابه
Activity of the Chlamydomonas chloroplast rbcL gene promoter is enhanced by a remote sequence element.
The chloroplast gene rbcL encodes the large subunit of ribulose bisphosphate carboxylase. In Chlamydomonas reinhardtii, this gene is transcribed more actively than any other protein-encoding chloroplast gene studied to date. To delineate the rbcL gene promoter, chimeric reporter genes containing fragments of the 5' region of the rbcL gene fused to the coding sequence of the bacterial uidA gene,...
متن کاملChloroplast DNA replication is regulated by the redox state independently of chloroplast division in Chlamydomonas reinhardtii.
Chloroplasts arose from a cyanobacterial endosymbiont and multiply by division. In algal cells, chloroplast division is regulated by the cell cycle so as to occur only once, in the S phase. Chloroplasts possess multiple copies of their own genome that must be replicated during chloroplast proliferation. In order to examine how chloroplast DNA replication is regulated in the green alga Chlamydom...
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15 صفحه اولExtensive sequence divergence in the 3' inverted repeat of the chloroplast rbcL gene in non-flowering land plants and algae.
A stem-loop region is present at the 3' terminus of the chloroplast rbcL mRNA in all taxa surveyed to date. In spinach, this structure has been shown by others to be involved in modulating transcript stability and correct 3' terminus processing, and is a conserved feature of other flowering plant rbcL mRNAs. In Chlamydomonas reinhardtii, an analogous structure has been shown by others to serve ...
متن کاملTranscriptional analysis of endogenous and foreign genes in chloroplast transformants of Chlamydomonas.
Transcription from modified chloroplast genes has been studied in vitro, but only with the recently developed ability to stably introduce foreign DNA into Chlamydomonas reinhardtii chloroplast chromosomes in situ has it become possible to do so in vivo. Cloned chloroplast DNA sequences, into which had been inserted chimeric genes composed of the GUS coding sequence reporter under transcriptiona...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.23.10819